WARNING: The following article contains spoilers for Sony's Spider-Man: Into the Spider-Verse, in theaters now.

With Spider-Man: Into the Spider-Verse starring a whole host of web-slinging superheroes from across the multiverse, it's only natural the acclaimed animated film similarly features a robust rogues' gallery.

The villains are, for the most part, based on their comic book counterparts in Marvel's Ultimate Universe, assembled in the film's version of the Sinister Six (although that name is never used). Below is a rundown of the colorful Spider-Man foes who make a full appearance in Into the Spider-Verse, alongside a nefarious organization with its own comic book ties.

Green Goblin

Spider-Man: Into the Spider-Verse

The first villain fully introduced in the film is Norman Osborn's Green Goblin. Seen battling the Peter Parker of Miles Morales' universe inside the Brooklyn-based Alchemax facility, this incarnation of the Green Goblin is the largest and most monstrous version to appear yet. Towering over Spider-Man like an amateur kaiju, the transformed Norman features scaly skin, leathery wings, and a tail while flinging fireballs at his foe.

RELATED: Spider-Man: Into the Spider-Verse's Ending, Explained

First created by Stan Lee and Steve Ditko in Amazing Spider-Man #14, the classic Marvel villain appears more similarly to the Ultimate Marvel character created by Brian Michael Bendis and Mark Bagley. Voiced by MacGruber director Jorma Taccone, the villain is defeated relatively early in the film though he reappears silently as Peter B. Parker from another universe explains his own superhero origins in comic book form.

Kingpin

Kingpin's animated face from the film Spider-Man: Into the Spider-Verse

The principal antagonist, Liev Schreiber's Wilson Fisk, runs the company Alchemax and employs the film's other villains. It's revealed Fisk is using the multiverse-threatening particle accelerator in a desperate bid to reunite with his dead wife Vanessa and their young son, who were killed in a car accident as they fled after discovering him fighting Spider-Man.

RELATED: The Spider-Mobile Debuts in New Into the Spider-Verse Clip

A mountain of a man, this version of Marvel's Kingpin not only is responsible for the Spider-heroes of the multiverse coming to his Earth but also plays a prominent role in Miles' own heroic origin story: In addition to funding the experiment that created the genetically enhanced spider that gave Miles his powers, Kingpin kills his universe's Spider-Man with his bare hands after he sabotages the particle accelerator. That act reminds audiences the villain was initially one of Spider-Man's worst enemies years before his association with Daredevil.

Tombstone

Tombstone picks his teeth at Kingpin's gala in Into The Spider-Verse

Depicted in Into the Spider-Verse as the Kingpin's top enforcer, Tombstone is rarely seen far from his boss' side. Often sporting matching twin pistols, the gray villain is voiced by Black Lightning actor Marvin "Krondon" Jones III. It's unknown whether he possesses the same levels of superhuman strength and endurance as his comic book counterpart, as he's effectively floored in the film from a blow by Aunt May.

The hard-hitting villain was introduced in 1988 in Web of Spider-Man #36 by Gerry Conway and Alex Saviuk, and had a memorable appearance earlier this year in Marvel's Spider-Man video game. By the end of the film, Tombstone is defeated along with his boss, and is seen being put into the back of a patrol car by police officer, and Miles' father, Jefferson Davis-Morales.

NEXT PAGE: One of the Villains Is Personal For Miles Morales

Prowler

spider-verse-prowler

The most personal enemy for Miles, and arguably most fearsome, Prowler is introduced as a deadly threat who often lurks in the shadows, and sports a set of sharp metal claws that can extend into lasers. Over the course of the film, Miles discovers the villain is actually his uncle Aaron Davis, who became estranged from his brother Jeff after turning to a life of crime.

RELATED: Spider-Man: Into the Spider-Verse Has the Perfect Shoutout to Donald Glover

This incarnation is the film's most direct adaptation of a villain, drawing inspiration from the Ultimate version of the character introduced in 2011 in Ultimate Comics: Spider-Man #1 by Brian Michael Bendis and Sara Pichelli. Voiced by Mahershala Ali, this version is slightly more noble than his comic book counterpart, refusing to kill Miles after discovering his nephew is the new Spider-Man, only to be murdered himself by Kingpin. His death casting a heavy shadow over Miles and his father for the remainder of the film.

Alchemax

While not a villain, per se, Alchemax appears prominently as a nefarious company run by Wilson Fisk to develop the particle accelerator. Much of the action takes place in company's testing facility in Brooklyn, but Miles and Peter also break into a compound in the Hudson Valley to obtain more data about the accelerator, which leads to a spirited chase by trigger-happy scientists after they catch Peter stealing a bagel from the cafeteria.

The organization was introduced by Peter David and Rick Leonardi in Spider-Man 2099 #1 as a mega corporation controlling virtually every aspect of consumer life in the United States. The company was later brought into the Marvel Universe's relative present day by Dan Slott and Ryan Stegman in Superior Spider-Man #19 after a merger by Oscorp and Horizon Labs. Given one character's appearance in the film, Into the Spider-Verse will likely not be the last audiences see of the company.

Doc Ock

Spider-Man: Into the Spider-Verse

The most original reimagining of any character is the film's version of longtime Spider-Man foe Doctor Octopus. Introduced as Olivia Octavius, the villain is the lead scientist on the particle accelerator project for Alchemax due to her advanced theories on the existence of the multiverse. After catching Peter Parker in her office attempting to steal data on the project, she reveals herself as Miles' universe incarnation of the classic villain, with her own set of robotic arms.

RELATED: New Spider-Man: Into the Spider-Verse Clip Introduces Spider-Gwen

While the original Doctor Octopus was introduced by Stan Lee and Steve Ditko in The Amazing Spider-Man #3, a female version known as Lady Octopus, or Doctor Octopus II, debuted in The Amazing Spider-Man #406, by J.M. DeMatteis and Angel Medina. Voice by Kathryn Hahn, Into the Spider-Verse's Doc Ock is ultimately taken down by a runaway bus in the extra-dimensional vortex unleashed by her own accelerator.

Scorpion

The last villain to receive a full introduction in the film, Scorpion joins the fight after tracking Miles to Aunt May's home in Queens. More cybernetically enhanced than traditional depictions, this version features a powerful claw arm and piercing tail, and can swap out his legs from a full robotic array befitting his arachnid namesake. Voiced by Joaquin Cosio, this version of the character also appears to be a native Spanish speaker, heard conversing in the language, with translations provided by handy panel on screen.

Introduced by Stan Lee and Steve Ditko in The Amazing Spider-Man #19, this incarnation of the villain puts up a strong fight in the finale, using his tail to disable Peni Parker's trusty SP//dr mech before being dispatched by Spider-Man Noir and Spider-Ham.

Directed by Bob Persichetti, Peter Ramsey and Rodney Rothman, Spider-Man: Into the Spider-Verse stars Shameik Moore, Brian Tyree Henry, Jake Johnson, Mahershala Ali, Hailee Steinfeld, Liev Schreiber, Luna Lauren Velez, Lily Tomlin, Nicolas Cage, John Mulaney and Kimiko Glenn. The film is in theaters now.