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WATCH: Batgirl Meets the TMNT in Batman Vs. Teenage Mutant Ninja Turtles

The Dark Knight and Heroes in a Half-Shell will face off in the upcoming animated movie Batman vs. Teenage Mutant Ninja Turtles, and Warner Bros. and Nickelodeon have released the opening scene from the eagerly anticipated film.

Showing Batgirl's first meeting with the Ninja Turtles, the scene begins with Barbara Gordon visiting a power generator when the facility is attacked by deadly ninjas.

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Outnumbered but undeterred, the Teenage Mutant Ninja Turtles leap into action through a disorienting smoke bomb and quickly dispatch all of their enemies before Barbara can take them on herself. When the smoke clears, Barbara discovers the generator has been stolen and briefs Batman on the situation.

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"Whatever they are, they're going to regret setting foot in Gotham," the Dark Knight vows after hearing about Batgirl's encounter.

RELATED: Batman Vs. Teenage Mutant Ninja Turtles Trailer Teases Deadly Alliances

Batman vs. Teenage Mutant Ninja Turtles is scheduled to be released on May 14 on Blu-ray, 4K UHD and Digital HD. It is voiced by Troy Baker as Batman and the Joker, Darren Criss as Raphael, Kyle Mooney as Michelangelo, Baron Vaughn as Donatello, Eric Bauza as Leonardo and Rachel Bloom as Batgirl.

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