Warner Bros. Animation and DC Entertainment have released a new still from their upcoming animated film, "Batman: The Killing Joke," featuring Batgirl.

Written by Alan Moore and illustrated by Brian Bolland, "The Killing Joke" is one of the most iconic and acclaimed Joker stories ever published, along with one of the most controversial, primarily stemming from its depiction of brutal violence against Barbara Gordon.

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In the adaptation, Barbara's time as the costumed Batgirl will be seen in a prologue sequence. Though Barbara was an important part of the original graphic novel's story, her superhero career was never explored. Actor Tara Strong, who voiced Barbara/Batgirl in numerous television series, animated features and video games dating back to 1997's "The New Batman Adventures," will reprise her role as the character.

What does it take for a person to snap? How much despair for a mind to fracture? These are the twisted questions that The Joker has set out to answer, to show Gotham that even an ordinary man like Commissioner Gordon is only one bad day away from sheer insanity. Based on the acclaimed DC Comics graphic novel, witness a journey into the dark psyche of the Clown Prince of Crime. Follow his humble beginnings as a struggling comic to his fateful encounter with the Dark Knight that changed everything. Fueled by the return of Kevin Conroy as Batman and Mark Hamill as The Joker, witness the birth of a super villain, the fortitude of a hero and the punch line that will leave you speechless.

WATCH: 12-Minute Sneak Peek of "Batman: The Killing Joke" Animated Movie

The first trailer for "The Killing Joke," which reunites "Batman: The Animated Series" voice actors Kevin Conroy and Mark Hamill, dropped in late April. The film will be rated R.

Based on Alan Moore and Brian Bolland's famed comic of the same name, and featuring the voices of Kevin Conroy (Batman), Mark Hamill (The Joker), Tara Strong (Batgirl) and Ray Wise (Commissioner Gordon), "Batman: The Killing Joke" is set to premiere at Comic-Con International in San Diego this July, with Digital HD release on July 26, and Blu-ray & DVD set for release on August 2.