43. Batwoman (Katherine Kane) - 311 points (2 first place votes)
Batwoman (created by Greg Rucka, Geoff Johns, Grant Morrison and Mark Waid) was introduced in 52, a storyline that was based around the notion that Batman, Superman and Wonder Woman were gone for a year. Thus, when Batwoman shows up in Gotham City there IS no Batman there...
Kate Kane grew up in a rich family but also a military family (her mom was a socialite and her dad was a Colonel). She was kicked out of a military academy because of Don't Ask Don't Tell. She spiraled out of control for a while there and was just a wild socialite. However, after Batman tried to save her from a mugger (that she managed to take out on her own), she was inspired to become a vigilante. Eventually her father discovered her activities and decided to help her, as well as provide her with a fancy Bat-suit. She has since become one of the most successful heroes in Gotham City.
Batwoman is one of the rare heroes where the New 52 didn't really change her at all.
42. Starman (Jack Knight) - 312 points (9 first place votes)
Jack Knight was created by James Robinson and Tony Harris as the unlikely wielder of the name Starman and the famous cosmic rod of Starman.
When Ted Knight chose to retire, his son David was the clear choice. He WANTED to be a hero. Jack did not.
But when David was murdered, Jack had to step up and become the new Starman, which he acquitted himself mightily at for many years.
Jack was interesting in particular because he WASN'T your typical hero, so he brought an outsider's perspective to the whole thing which was very engaging. He dealt in pop culture antiquities, so it allowed Robinson to have Jack make references most superheroes would never make. Another striking aspect of the series was Jack's relationship with the "villain" the Shade. Their friendship was awesome, as it sort of packaged everything about what made Starman great into one thing - Jack's out-of-the-box thinking as a hero, his off-kilter cultural references and, well, the Shade (the Shade was great).
Continuing in his unconventional way, Jack retired from being a superhero when he had his first child. That meant that he missed out on the New 52, which...well, yeah. Let's leave it at that.
41. Dr. Fate (Kent Nelson) - 319 points (5 first place votes)
Created by Gardner Fox and Howard Sherman, Kent Nelson discovered a sorcerer named Nabu, who then trained Kent to become Doctor Fate, complete with his magic helmet.
I always am partial to how Fox and Murphy Anderson brought Fate into the Silver Age...
Many other character have taken on the iconic helmet in the years since, most recently in the New 52 a young medical student named Khalid Nassour has become the new Doctor Fate.
Okay, that's it for the first batch of characters!