Warner Bros' first R-rated animated feature film, "Batman: The Killing Joke," took in a sizable haul during its special two-night theatrical run this week, grossing $3.8 million in ticket sales, according to Variety.

Despite the film's controversial subject matter, it was the biggest exclusive release from Fathom Events, which usually focuses on live-streaming for theater chains. 1325 locations showed "The Killing Joke" on Monday night, raking in an average of $2,396 per site, with 810 theaters earning about $800,000 total on Tuesday. Monday night's success even managed to supplant "Star Trek: Beyond," which earned about $1,592 per theater.

With Kevin Conroy and Mark Hamill reprising their respective animated roles as Batman and the Joker, "The Killing Joke" is based on the 1988 graphic novel written by Alan Moore with art by Brian Bolland, and focuses on the origins of the Dark Knight's arch-nemesis while laying the groundwork for Barbara Gordon's later transformation into Oracle following a horrific attack from the Clown Prince of Crime.

For those who missed the theater run, "Batman: The Killing Joke" is available on VOD now, and will be released on Blu-ray and DVD on August 2.